Scientists Construct Integrated Gene Logic-Chips Called ‘Gene Nanochips’
August 21, 2018 | Osaka UniversityEstimated reading time: 2 minutes
Gene expression is a fundamental of life, where each cell switches on and off specific genes. Thus, an autonomous device that could control the on-off switching would have great value in medical care.
Synthetic genetic circuits are a technology to control gene expression and program cells to perform desired functions. Therefore, increasing the complexity of the genetic circuit will allow us to control cell fates more accurately.
However, the complexity of genetic circuits remains low. This is because, in conventional reaction-diffusion systems, the enzymes and substrates are provided separately, and non-specific binding of the enzymes to the substrates causes unintended crosstalk among the different circuits.
Osaka University-led researchers, in a joint research project with The University of Tokyo, Kyoto University, and Waseda University, constructed integrated gene logic-chips called "gene nanochips." Using integrated factors on the nanochips, these self-contained nanochips can switch genes on and off within a single chip, preventing unintended crosstalk.
The researchers showed the autonomous responses of the nanochips in artificial cells: environmental sensing, information computation and product output at the single-chip level. Their research results were published in Nature Nanotechnology.
DNA nanotechnology is a versatile method used to construct custom structures and to control precise molecular layouts. The researchers used a rectangular sheet (90 nm wide, 60 nm deep, 2 nm high), and integrated enzyme, RNA polymerase (RNAP, an enzyme that synthesizes RNA from a DNA template), and multiple target-gene substrates.
The nano-layout ability of DNA nanotechnology allows the researcher to rationally design gene expression levels by changing the intermolecular distances between the enzyme and the target genes, thus affecting the collision efficiency and subsequent reaction.
The researchers further integrated sensors. Ideally, a sensor that is capable of detecting any type of signal should have minimal design limitations. However, conventional methods have suffered from several limitations (e.g., materials). This is because, in conventional genetic circuits, the sensor is part of the substrate of the enzyme (e.g., DNA in transcription).
Conversely, in this study, the sensor part was independent of the enzymatic reaction. Thus, the researchers can use any sensor materials that change the effective intermolecular distance on signal recognition, allowing the construction of various sensors responding to distinct signals (microRNAs, chemical compounds, proteins and light). Moreover, by combining and integrating sensors responding to distinct signals, the researchers have succeeded in photo-reprogramming of the genetic circuits.
Finally, the nanochip allowed the researchers to simplify the construction of a genetic circuit that responded to an artificial cell, a water-in-oil droplet, and they could compute its miRNA profile, by simply mixing the orthogonal chips, expanding the power of the genetic circuit.
Corresponding author Hisashi Tadakuma says, “All factors necessary for transcription reactions are on this integrated nanochip, so environmental sensing, information computation, and product output can be completed at the single-chip level. In the near future, autonomous nanochips will be useful in maintaining the cell in the healthy state through controlling gene expression spatially and temporally, which will embody the ideal of the saying ‘prevention is best cure’. ”
Suggested Items
Zentech’s Board of Directors Announces the Return of Matt Turpin as President and CEO
04/22/2024 | Zentech ManufacturingTurpin draws upon over 35 years of experience in the electronics industry and has an 18-year history with Zentech. He previously served as President and CEO from 2006 to 2019 after which time he has remained active in the EMS industry as an advisor to Zentech and other industry organizations.
SEMI Talent Forum 2024 to Help Build Next-Generation Chip Industry Workforce
04/09/2024 | SEMIPost-graduates and onboarding talent will connect with local companies to explore semiconductor industry career paths and employment opportunities at the SEMI Talent Forum, May 1, 2024 at the University of Swansea.
Indium Corporation Technical Manager to Present at SMTA Taiwan Tech Forum
03/12/2024 | Indium CorporationIndium Corporation Senior Area Technical Manager Jason Chou will present at the SMTA Taiwan Tech Forum on March 26 in Taoyuan City, Taiwan. Chou’s presentation will highlight the growing demand for high reliability and low-temperature solder solutions brought on by the rapidly developing electric vehicle (EV) and artificial intelligence (AI) industries.
Fujitsu, Delft University of Technology Establish New Quantum Lab
01/26/2024 | JCN NewswireFujitsu and Delft University of Technology today announced the establishment of the Fujitsu Advanced Computing Lab Delft at Delft University of Technology, an industry-academia collaboration hub dedicated to the development of quantum computing technologies.
Foundations of the Future: Awarding Scholarships and Awards in 2023
01/24/2024 | IPC Education Foundation -- Column: Foundations of the FutureProviding scholarships and awards to hard-working students who excel academically, demonstrate a commitment to electronics manufacturing, and are active members of the IPC student network, supports the overall mission of the IPC Education Foundation, which is to develop a talent pipeline for the industry.